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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [2]
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- Product number
- MA5-24155 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPR34 Monoclonal Antibody (419859)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody stains human GPR34 transfectants but not irrelevant transfectants. Reconstitute at 0.5 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 419859
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of GPR34 in immersion fixed A172 human glioblastoma cell line. Samples were incubated in GPR34 monoclonal antibody (Product # MA5-24155) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of A172 human glioblastoma cell line was stained with mouse Anti-human GPR34 Monoclonal Antibody (Product # MA5-24155) or isotype control antibodyopen histogram), followed by Phycoerythrin-conjugated Anti-mouse IgG F (ab')2 Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of GPR34 in A172 human glioblastoma cell line. Samples were incubated in GPR34 monoclonal antibody (Product # MA5-24155) or isotype control antibody followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab)2 Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
