AP5752A

antibody from Abgent

Targeting: TUBB3 beta-4, CFEOM3, CFEOM3A, FEOM3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP5752A

Provider

Abgent

Proper citation

Abgent Cat#AP5752a, RRID:AB_10817391

Product name

TUBB3 Antibody (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

TUBB3 Antibody (N-term) (Cat. #AP5752a) western blot analysis in mouse brain tissue lysates (15ug/lane).This demonstrates the TUBB3 antibody detected the TUBB3 protein (arrow).

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

TUBB3 Antibody (N-term) (Cat. #AP5752a) western blot analysis in HepG2 cell line lysates (15ug/lane).This demonstrates the TUBB3 antibody detected the TUBB3 protein (arrow).

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

TUBB3 Antibody (N-term) (Cat. #AP5752a) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TUBB3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:10~50

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

TUBB3 Antibody (N-term) (Cat. #AP5752a) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50