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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [6]
- Immunocytochemistry [3]
- Immunoprecipitation [1]
- Immunohistochemistry [5]
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- Product number
- GTX631836 - Provider product page
- Provider
- GeneTex
- Product name
- beta Tubulin 3/ Tuj1 antibody [GT11710]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
Submitted references Generation and quantitative proteomics analysis of CK2α/α'(-/-) cells.
Sambucus williamsii induced embryonic stem cells differentiated into neurons.
Borgo C, Franchin C, Scalco S, Bosello-Travain V, Donella-Deana A, Arrigoni G, Salvi M, Pinna LA
Scientific reports 2017 Feb 17;7:42409
Scientific reports 2017 Feb 17;7:42409
Sambucus williamsii induced embryonic stem cells differentiated into neurons.
Liu SP, Hsu CY, Fu RH, Huang YC, Chen SY, Lin SZ, Shyu WC
BioMedicine 2015;5(1):3
BioMedicine 2015;5(1):3
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Supportive validation
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- Experimental details
- beta III Tubulin antibody detects beta III Tubulin protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with beta III Tubulin antibody (GTX631836) diluted by 1:1000.
- Validation comment
- WB
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- Experimental details
- beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by western blot analysis. Various whole cell extracts (20 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at a dilution of 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:200000.
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- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (10 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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- Experimental details
- Wild-type (WT) and beta Tubulin 3/ Tuj1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:2500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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- Main image
- Experimental details
- Wild-type (WT) and beta Tubulin 3/ Tuj1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:2500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
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- Experimental details
- beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein expression by immunofluorescent analysis.Sample: Cultured rat E18 primary hippocampal neuron. Cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).
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- Experimental details
- beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein at cytoskeleton by immunofluorescent analysis.Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Hoechst 33342 staining.
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- beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein at cytoskeleton by immunofluorescent analysis.Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.Green: NAGLU protein stained by NAGLU antibody [N3C1], Internal (GTX114569) diluted at 1:200.Blue: Hoechst 33342 staining.
Supportive validation
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- Immunoprecipitation of beta III Tubulin protein from SK-N-SH whole cell extracts using 5 £gg of beta III Tubulin antibody [GT11710] (GTX631836).Western blot analysis was performed using beta III Tubulin antibody [GT11710] (GTX631836).EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
Supportive validation
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- Experimental details
- beta III Tubulin antibody [GT11710] detects beta III Tubulin proteins on embryonic mouse brain by immunohistochemical analysis. Sample:Frozen section of embryonic mouse brain (mE18.5). Red: beta III Tubulin antibody [GT11710] (GTX631836) diluted at 1:500. Blue: DAPI
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- beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
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- beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein by immunohistochemical analysis.Sample: Frozen sectioned E13.5 rat brain.Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250.Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX131836) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein by immunohistochemical analysis.Sample: Frozen sectioned E13.5 rat brain. Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX131836) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).
