ARG53750

antibody from Arigo

Targeting: TUBB3 beta-4, CFEOM3, CFEOM3A, FEOM3

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

ARG53750

Provider

Arigo

Product name

anti-beta III Tubulin antibody [TU-20] (FITC)

Antibody type

Monoclonal

Antigen

Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.

Host

Mouse

Conjugate

Green dye

Isotype

IgG

Antibody clone number

TU-20

Vial size

100 µg

Concentration

0.1 mg/ml

Storage

Aliquot and store in the dark at 2-8°C. Do not freeze. Keep protected from prolonged exposure to light. Short-term exposure to room temperature should not affect the quality of the reagent.

Handling

The antibody solution should be gently mixed before use.

Immunocytochemistry

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunofluorescence: Neuro2a mouse neuroblastoma cell stained with clone TU-20 (green) Cell nuclei was stained with DAPI (blue).

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunofluorescence: P-19 mouse embryonal carcinoma cells stimulated to neuronal differentiation by retinoic acid stained with clone TU-20 (red) Cell nuclei was stained with DAPI (blue).

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunofluorescence: P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid co-stained with stained with clone TU-20 (red) and anti-beta-tubulin (green) Superposition of red and green colours provided yellow staining. Nuclei were stained with DNA-binding dye (blue).