NB600-1018

antibody from Novus Biologicals

Targeting: TUBB3 beta-4, CFEOM3, CFEOM3A, FEOM3

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB600-1018

Provider

Novus Biologicals

Proper citation

Novus Cat#NB600-1018, RRID:AB_10001615

Product name

Mouse Monoclonal beta-III Tubulin Antibody

Antibody type

Monoclonal

Description

Ammonium sulfate precipitation. The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.

Reactivity

Human, Mouse, Rat, Canine

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

BRI2 Processing and Its Neuritogenic Role Are Modulated by Protein Phosphatase 1 Complexing.

Martins F, Serrano JB, Müller T, da Cruz E Silva OAB, Rebelo S
Journal of cellular biochemistry 2017 Sep;118(9):2752-2763

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Presence of intramucosal neuroglial cells in normal and aganglionic human colon.

Badizadegan K, Thomas AR, Nagy N, Ndishabandi D, Miller SA, Alessandrini A, Belkind-Gerson J, Goldstein AM
American journal of physiology. Gastrointestinal and liver physiology 2014 Nov 15;307(10):G1002-12

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Chronic exposure of adult rats to low doses of methylmercury induced a state of metabolic deficit in the somatosensory cortex.

Kong HK, Wong MH, Chan HM, Lo SC
Journal of proteome research 2013 Nov 1;12(11):5233-45

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Chronic effects of pyridoxine in the gerbil hippocampal CA1 region after transient forebrain ischemia.

Yoo DY, Kim W, Nam SM, Chung JY, Choi JH, Yoon YS, Won MH, Hwang IK
Neurochemical research 2012 May;37(5):1011-8

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A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional regulator of neuronal differentiation.

Theodorou E, Dalembert G, Heffelfinger C, White E, Weissman S, Corcoran L, Snyder M
Genes & development 2009 Mar 1;23(5):575-88

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Expression of class III beta-tubulin in colorectal carcinomas: an immunohistochemical study using TU-20 & TuJ-1 antibody.

Jirásek T, Cipro S, Musilová A, Kubecová M, Mandys V
The Indian journal of medical research 2009 Jan;129(1):89-94

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Expression of class III beta-tubulin in malignant epithelial tumours: an immunohistochemical study using TU-20 and TuJ-1 antibodies.

Jirásek T, Písaríková E, Viklický V, Mandys V
Folia histochemica et cytobiologica 2007;45(1):41-5

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Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells.

Katsetos CD, Dráberová E, Smejkalová B, Reddy G, Bertrand L, de Chadarévian JP, Legido A, Nissanov J, Baas PW, Dráber P
Neurochemical research 2007 Aug;32(8):1387-98

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Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells.

Kukharskyy V, Sulimenko V, Macůrek L, Sulimenko T, Dráberová E, Dráber P
Experimental cell research 2004 Aug 1;298(1):218-28

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Differential subcellular distribution of tubulin epitopes in boar spermatozoa: recognition of class III beta-tubulin epitope in sperm tail.

Pĕknicová J, Kubátová A, Sulimenko V, Dráberová E, Viklický V, Hozák P, Dráber P
Biology of reproduction 2001 Sep;65(3):672-9

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Differential subcellular distribution of tubulin epitopes in boar spermatozoa: recognition of class III beta-tubulin epitope in sperm tail.

Pĕknicová J, Kubátová A, Sulimenko V, Dráberová E, Viklický V, Hozák P, Dráber P
Biology of reproduction 2001 Sep;65(3):672-9

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Expression of class III beta-tubulin in normal and neoplastic human tissues.

Dráberová E, Lukás Z, Ivanyi D, Viklický V, Dráber P
Histochemistry and cell biology 1998 Mar;109(3):231-9

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Expression of class III beta-tubulin in normal and neoplastic human tissues.

Dráberová E, Lukás Z, Ivanyi D, Viklický V, Dráber P
Histochemistry and cell biology 1998 Mar;109(3):231-9

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: beta-III Tubulin Antibody (TU-20) [NB600-1018] - An intracellular stain was performed on SH-SY5Y cells with beta-III Tubulin (TU-20) antibody NB600-1018PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: beta-III Tubulin Antibody (TU-20) [NB600-1018] - Analysis of PerCP conjugate of NB600-1018. An intracellular stain was performed on SH-SY5Y cells with beta-III Tubulin (TU-20) antibody NB600-1018PCP (blue) and a matched isotype control NBP2-27287PCP (orange). Cells were fixed with 4% PFA and then permea