657412

antibody from BioLegend

Targeting: TUBB3 beta-4, CFEOM3, CFEOM3A, FEOM3

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Immunoelectron microscopy (Supportive data in Antibodypedia)

Antibody Data

Product number

657412

Provider

BioLegend

Proper citation

BioLegend Cat#657412, RRID:AB_2632699

Product name

Brilliant Violet 421™ anti-Tubulin Beta 3 (TUBB3)

Antibody type

Monoclonal

Description

Tubulin is the main component of microtubules. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 (TUBB3) is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Blue dye

Antibody clone number

AA10

Vial size

100 µg

Concentration

0.2 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunoelectron microscopy

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human lung adenocarcinoma cell line A549 was fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5 % Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then, the tissue was stained with 2.5 µg/ml of Brilliant Violet 421™ anti-Tubulin Beta 3 (TUBB3) (clone AA10, blue) overnight at 4°C. Nuclei were counterstained with DRAQ5™ (red). The image was captured with a 40X objective.

Conjugate

Blue dye