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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- EB06339 - Provider product page
- Provider
- Everest Biotech
- Proper citation
- Everest Biotech Cat#EB06339, RRID:AB_2121959
- Product name
- Goat anti-CMG1 / CCDC2 / IFT74 Antibody
- Antibody type
- Polyclonal
- Description
- Goat anti-CMG1 / CCDC2 / IFT74 Antibody
- Reactivity
- Human
- Host
- Goat
- Antigen sequence
C-KTIVDALHSTSGN- Epitope
- C Terminus
- Isotype
- IgG
- Vial size
- 100µg specific antibody in 200µl
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store at -20°C. Minimize freezing and thawing.
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Supportive validation
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- EB06339 staining (0.3µg/ml) of 293 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- A) EB06339 (2.5mg/ml) staining of primary rat cortical neurons showed localization of IFT74 to vesicles in the cell body and along the neuronal processes. B) Control. (Data were kindly provided by Dr. Bryan Traynor.)
Supportive validation
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- EB06339 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Figure 2: EB06339 (2.5mg/ml) staining of primary rat cortical neurons showed localization of IFT74 to vesicles in the cell body and along the neuronal processes. B) Control. (Data were kindly provided by Dr. Bryan Traynor.)
