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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- ARG63497 - Provider product page
- Provider
- Arigo
- Product name
- anti-CMG1 / CCDC2 / IFT74 antibody
- Antibody type
- Polyclonal
- Antigen
- C-KTIVDALHSTSGN
- Description
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Reactivity
- Human, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: 293 lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63497 anti-CMG1 / CCDC2 / IFT74 antibody at 0.3 µg/ml dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: primary rat cortical neurons stained with A) ARG63497 anti-CMG1 / CCDC2 / IFT74 antibody (2.5mg/ml) shows localization of IFT74 to vesicles in the cell body and along the neuronal processes. B) Control. (Data were kindly provided by Dr. Bryan Traynor.)
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin embedded Human Liver. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63497 anti-CMG1 / CCDC2 / IFT74 antibody at 5 µg/ml dilution followed by AP-staining. Figure 2: ARG63497 (2.5mg/ml) staining of primary rat cortical neurons showed localization of IFT74 to vesicles in the cell body and along the neuronal processes. B) Control. (Data were kindly provided by Dr. Bryan Traynor.)
