Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
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Validation data
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- Product number
- LS-C742553 - Provider product page
- Provider
- LSBio
- Product name
- IFT74 / CCDC2 Antibody (C Terminus, Biotin) LS-C742553
- Antibody type
- Polyclonal
- Description
- ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Storage
- Aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IFT74 / CCDC2 biotinylated antibody (0.2µg/ml) staining of HEK293 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitue for skimmed milk.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IFT74 / CCDC2 biotinylated antibody (0.2µg/ml) staining of HEK293 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitue for skimmed milk.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IFT74 / CCDC2 biotinylated antibody (0.2µg/ml) staining of HEK293 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitue for skimmed milk.