PA5-24082

antibody from Invitrogen Antibodies

Targeting: DEGS2 C14orf66, DES2, FADS8

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-24082

Provider

Invitrogen Antibodies

Product name

DEGS2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with bovine and mouse based on sequence homology.

Reactivity

Human

Host

Rabbit

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Cell metabolism regulates integrin mechanosensing via an SLC3A2-dependent sphingolipid biosynthesis pathway.

Boulter E, Estrach S, Tissot FS, Hennrich ML, Tosello L, Cailleteau L, de la Ballina LR, Pisano S, Gavin AC, Féral CC
Nature communications 2018 Nov 19;9(1):4862

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis using a DEGS2 polyclonal antibody (Product # PA5-24082) in HeLa cell lysates (35 µg per lane).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human kidney tissue using a DEGS2 polyclonal antibody (Product # PA5-24082), followed by HRP-conjugated secondary antibody and DAB staining.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 5 CD98hc determines the level of DES2 in fibroblasts. a - d Loss of CD98hc or expression of C330S mutant reduce the expression of DES2 and induces DES1. One experiment representative of at least n = 3 experiments. e - h Levels of DES1 and DES2 mRNA as measured by RT-qPCR on total mRNA from CD98hc- cells or C330S reconstituted cells. Means are plotted with s.e.m. as error bars from, respectively, n = 6 or n = 3 experiments. Differences were not statistically different in a Student's t test. i Schematic representation of the de novo sphingolipid biosynthesis pathway presenting variations of metabolites. Numbers refer to metabolites depicted and numbered in the volcano plot of Fig. 4d . Metabolites and p values are reported in Supplementary Data 1 . P values were calculated using a Student's t test. DES1/2 variations were extracted from results presented in Fig. 5a-d and Supplementary Figure 5A-D . j , k Hsp90 inhibition in control cells reduces the level of DES2 expressed in cells. Right panel, means are plotted with s.e.m. as error bars from n = 3 experiments, ** P < 0.01 in a Student's t test. l Grp78 and Grp94 co-immunoprecipitate with DES2 upon loss of CD98hc or geldanamycin treatment. Control, CD98hc-, or C330S-expressing cells were treated with geldanamycin for 16 h prior to cell lysis and DES2 immunoprecipitation. Precipitates were loaded on SDS-PAGE and analyzed by Western blot. One experiment representative of n = 2