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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-35053 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PNN Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine and mouse based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.32 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PNN in NCI-H460 cell lysate (35 µg/lane) using a PNN polyclonal antibody (Product # PA5-35053).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PNN in NCI-H460 cells using a PNN polyclonal antibody (Product # PA5-35053) followed by detection using a fluorescent conjugated secondary antibody (green). Nuclei were stained with Dapi (blue) and cytoplasmic actin was stained with a fluorescent phalloidin (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PNN in formalin-fixed, paraffin-embedded human liver tissue using a PNN polyclonal antibody (Product # PA5-35053) followed by peroxidase conjugation of the secondary antibody and DAB staining.
