AMAb91528

antibody from Atlas Antibodies

Targeting: TMEM119 OBIF

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

AMAb91528

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#AMAb91528, RRID:AB_2797214

Product name

Anti-TMEM119

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

GDGARMVEGRGAEEEEKGSQEGDQEVQGHGVPVET
PEAQEEPCSGVLEGAVVAGEGQGELEGSLLLAQEA
QGPVGPPESPCACSSVHPS


Isotype

IgG

Antibody clone number

CL8714

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line SH-SY5Y.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of SH-SY5Y cells using the Anti-TMEM119 monoclonal antibody, showing specific staining to the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Sample type

HUMAN

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human cerebral cortex and liver tissues using AMAb91528 antibody. Corresponding TMEM119 RNA-seq data are presented for the same tissues.

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows strong membranous positivity in microglia.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows no positivity in hepatocytes as expected.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebellum shows strong membranous positivity in microglia.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lymphoid tissues shows strong membranous positivity in germinal center cells.