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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [5]
- Flow cytometry [2]
- Other assay [1]
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- Product number
- PA5-85926 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD61 (Integrin beta 3) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references C/D box snoRNA SNORD113-6/AF357425 plays a dual role in integrin signalling and arterial fibroblast function via pre-mRNA processing and 2'O-ribose methylation.
van Ingen E, van den Homberg DAL, van der Bent ML, Mei H, Papac-Milicevic N, Kremer V, Boon RA, Quax PHA, Wojta J, Nossent AY
Human molecular genetics 2022 Mar 31;31(7):1051-1066
Human molecular genetics 2022 Mar 31;31(7):1051-1066
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD61 (Integrin beta 3) in A431 (1) and rat spleen tissue (2) lysate using a CD61 (Integrin beta 3) Polyclonal antibody (Product # PA5-85926) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD61 (Integrin beta 3) of paraffin-embedded rat ossature tissue using a CD61-Integrin-beta-3 Polyclonal antibody (Product #PA5-85926). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD61 (Integrin beta 3) of paraffin-embedded Human tonsil tissue using a CD61-Integrin-beta-3 Polyclonal antibody (Product #PA5-85926). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD61 (Integrin beta 3) of paraffin-embedded Human kidney tissue using a CD61-Integrin-beta-3 Polyclonal antibody (Product #PA5-85926). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD61 (Integrin beta 3) of paraffin-embedded Human placenta tissue using a CD61-Integrin-beta-3 Polyclonal antibody (Product #PA5-85926). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD61 (Integrin beta 3) of paraffin-embedded Mouse kidney tissue using a CD61-Integrin-beta-3 Polyclonal antibody (Product #PA5-85926). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of CD61 (Integrin beta 3) in A431 cells using a CD61 (Integrin beta 3) Polyclonal Antibody (Product # PA5-85926) at a dilution of 1:100, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of CD61 (Integrin beta 3) in HUVEC cells using a CD61 (Integrin beta 3) Polyclonal Antibody (Product # PA5-85926) at a dilution of 1:100, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Protein expression in murine and human primary fibroblasts. ( A ) Western blots for JAG1, DUSP7, ITGB3 and MAP2K1, each with GAPDH, of three independent replicates (each band represents a pool of three technical triplicates) in PMFs. ( B ) Western blots for JAG1, DUSP7, ITGB3 and MAP2K1, with GAPDH of three independent replicates (each band represents a pool of three technical triplicates) in HUAFs. ( C - L ) Quantifications of protein band intensities, normalized to GAPDH. Data are represented as mean +- SEM. * * P < 0.01, compared with GM-ctrl.
