Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- GTX25190 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX25190, RRID:AB_380481
- Product name
- Integrin beta 3 (phospho Tyr773) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Feline
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Melanoma cells transfected with human Integrin av and wild-type (WT) or mutant (Y773F) human Integrin beta3 were analyzed. Integrin beta3 was immunoprecipitated then incubated with Integrin beta3 [pY773] polyclonal antibody along with lysates corresponding to non-phosphorylated Integrin beta3 (1), phosphorylated WT Integrin beta3 (2), or phosphorylated Y773F mutant Integrin beta3 (3). Elimination of signal in site-directed mutant demonstrates antibody specificity.