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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- R30561 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- AQP3 Antibody Aquaporin 3
- Antibody type
- Polyclonal
- Antigen
- An amino acid sequence from the C-terminus of human Aquaporin 3 (EENVKLAHVKHKEQI) was used as the immunogen for this AQP3 antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- After reconstitution, the AQP3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of AQP3 antibody and Lane 1: rat kidney; 2: (r) lung; 3: mouse kidney; 4: human M453; 5: (h) SMMC cell lysate
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC testing of AQP3 antibody and HeLa cells
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: AQP3 antibody testing of rat kidney tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-F testing of AQP3 antibody and rat kidney tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: AQP3 antibody testing of human renal cancer tissue. HIER: Boil the paraffin sections in 10mM citrate buffer, pH6, for 20 mins.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: AQP3 antibody testing of human lung cancer tissue. HIER: Boil the paraffin sections in 10mM citrate buffer, pH6, for 20 mins.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human HeLa cells with AQP3 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=AQP3 antibody.
