A305-854A-T

antibody from Invitrogen Antibodies

Targeting: EFL1 EFTUD1, FAM42A, FLJ13119, HsT19294, RIA1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A305-854A-T

Provider

Invitrogen Antibodies

Product name

EFTUD1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse EFTUD1 by western blot. Samples: Whole cell lysate (50 µg) from RKO, Jurkat, A-549, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EFTUD1 antibody A305-854A-M (lot A305-854A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human EFTUD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from RKO cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EFTUD1 antibody (Product # A305-854A-T; lot A305-854A-T-1) used for IP at 100 µL per reaction. EFTUD1 was also immunoprecipitated by rabbit anti-EFTUD1 antibody (Product # A305-853A-T). For blotting immunoprecipitated EFTUD1, Product # A305-854A-T was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.