Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-28781 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- alpha TTP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: IMR32, mouse liver, rat liver.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of alpha TTP was performed by separating 50 µg of mouse tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a alpha TTP Polyclonal Antibody (Product # PA5-28781) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of alpha TTP was performed by separating 50 µg of rat tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a alpha TTP Polyclonal Antibody (Product # PA5-28781) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using alpha TTP Polyclonal Antibody (Product # PA5-28781). Sample (30 µg of whole cell lysate). Lane A: IMR32. 12% SDS PAGE. alpha TTP Polyclonal Antibody (Product # PA5-28781) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of alpha TTP in methanol-fixed MCF-7 cells using an Alpha TTP polyclonal antibody (Product # PA5-28781) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- alpha TTP Polyclonal Antibody detects TTPA protein at cytosol on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. Alpha TTP Polyclonal Antibody (Product # PA5-28781) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.