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LearnAAR-014-200UL
antibody from Invitrogen Antibodies
Targeting: ATP6AP2
APT6M8-9, ATP6IP2, ATP6M8-9, M8-9, PRR, RENR
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AAR-014-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Renin Receptor/ATP6AP2 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat heart membranes (lanes 1 and 4), mouse kidney membranes (lanes 2 and 5) and rat brain membranes (lanes 3 and 6): - 1-3. Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody (#AAR-014), (1:200). 4-6. Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody , preincubated with Renin Receptor/ATP6AP2 (extracellular) Blocking Peptide (#BLP-AR014).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and mouse BV-2 microglia cell line lysate (lanes 2 and 4): - 1, 2. Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody (#AAR-014), (1:200). 3, 4. Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody , preincubated with Renin Receptor/ATP6AP2 (extracellular) Blocking Peptide (#BLP-AR014).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat heart membranes (lanes 1 and 4), mouse kidney membranes (lanes 2 and 5) and rat brain membranes (lanes 3 and 6): - 1-3. Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody (#AAR-014), (1:200). 4-6. Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody , preincubated with Renin Receptor/ATP6AP2 (extracellular) Blocking Peptide (#BLP-AR014).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and mouse BV-2 microglia cell line lysate (lanes 2 and 4): - 1, 2. Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody (#AAR-014), (1:200). 3, 4. Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody , preincubated with Renin Receptor/ATP6AP2 (extracellular) Blocking Peptide (#BLP-AR014).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of Renin Receptor in rat cerebellum. Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody (#AAR-014), (1:300), followed by goat Anti-rabbit-AlexaFluor-488. A. Renin Receptor immunoreactivity (green) appears in neuronal profiles in the purkinje cell layer (arrows). B. Pre-incubation of the Antibody with Renin Receptor/ATP6AP2 (extracellular) Blocking Peptide (BLP-AR014), suppressed staining. Cell nuclei are stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of Renin Receptor in live intact human THP-1 monocytic leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody (#AAR-014), (2.5 µg) + goat- Anti-rabbit-FITC.
