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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- ELISA [4]
- Immunoprecipitation [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- NBP2-61469 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Monoclonal 5-Hydroxymethylcytosine Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: 5-Hydroxymethylcytosine Antibody (236) [NBP2-61469] - Analysis in mouse brain genomic DNA . The plate was directly coated with different concentrations of genomic DNA isolated from mouse brain tissue. 1 ug/mL or 3 ug/mL was used as the primary antibody, and a HRP conjugated anti-rabbit IgG as the secondary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: 5-hmC Antibody (RM236) [NBP2-61469] - Direct ELISA of Mouse brain genomic DNA using Anti-5-hmC antibody (NBP2-61469). The plate was directly coated with different concentrations of genomic DNA isolated from Mouse brain tissue. NBP2-61469 was used as the primary antibody, and a HRP conjugated Anti-Rabbit IgG as the secondary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: 5-hmC Antibody (RM236) [NBP2-61469] - ELISA of single stranded DNA using Anti-5-hmC antibody (NBP2-61469). The plate was coated with streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA. A serial dilution of NBP2-61469 was used as the primary antibody, and an alkaline phosphatase conjugated Anti-Rabbit IgG as the secondary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: 5-hmC Antibody (RM236) [NBP2-61469] - Direct ELISA of Mouse brain genomic DNA using Anti-5-hmC antibody (NBP2-61469). The plate was directly coated with different concentrations of genomic DNA isolated from Mouse brain tissue. NBP2-61469 was used as the primary antibody, and a HRP conjugated Anti-Rabbit IgG as the secondary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Methylated DNA Immunoprecipitation: 5-Hydroxymethylcytosine Antibody (236) [NBP2-61469] - Analysis was performed using 10:1 DNA:Ab ratio. 1 ng of unmethylated, 5-Methylcytosine (5-mC) or 5-Hydroxymethylcytosine (5-hmC) DNA standard (897 bp) was spiked in 1ug of genomic DNA isolated from HeLa cells as the control. Realtime PCR was then performed to determine the capture of DNA standard as in % of input.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Methylated DNA Immunoprecipitation: 5-hmC Antibody (RM236) [NBP2-61469] - hMeDIP was performed using Anti-5-hmC antibody (NBP2-61469) at a 10:1 DNA: Ab ratio. 1 ng of unmethylated, 5-Methylcytosine (5-mC) or 5-Hydroxymethylcytosine (5-hmC) DNA standard (897 bp) was spiked in 1ug of genomic DNA isolated from HeLa cells as the co
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: 5-Hydroxymethylcytosine Antibody (236) [NBP2-61469] - Staining of formalin fixed and paraffin embedded human brain tissue sections.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: 5-hmC Antibody (RM236) [NBP2-61469] - Immunohistochemical staining of formalin fixed and paraffin embedded Human brain tissue sections, using Rabbit monoclonal Anti-5-hmC (NBP2-61469) antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: 5-hmC Antibody (RM236) [NBP2-61469] - Flow Cytometry analysis of 5-hmC expression in HEK293 cells using NBP2-61470. The cells were fixed with ice-cold MeOH, permeabilized with 0.5% Triton X-100, denatured with 2N HCl, then stained with NBP2-61469 (Blue) or with a negative control antibody NBP
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Dot Blot: 5-hmC Antibody (RM236) [NBP2-61469] - Dot blot of double stranded DNA using Anti-5-hmC antibody (NBP2-61469). The membrane was pre-spotted with double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and unmethylated DNA.
