A305-867A-T

antibody from Invitrogen Antibodies

Targeting: HMCES C3orf37, DC12, SRAPD1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A305-867A-T

Provider

Invitrogen Antibodies

Product name

HMCES Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human HMCES by western blot. Samples: Whole cell lysate (50 µg) from MCF-7, Jurkat, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-HMCES antibody A305-867A-M (lot A305-867A-M-1) used for WB at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 minutes.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human HMCES by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-HMCES antibody (Product # A305-867A-T; lot A305-867A-T-1) used for IP at 40 µL per reaction. HMCES was also immunoprecipitated by rabbit anti-HMCES antibody (Product # A305-681A-T). For blotting immunoprecipitated HMCES, Product # A305-867A-T was used at 1:100. Detection: Chemiluminescence with an exposure time time of 3 seconds.