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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF3049 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal Carbonic Anhydrase VA/CA5A Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Carbonic Anhydrase VA/CA5A in direct ELISAs and Western blots. In direct ELISAs, approximately 4% cross-reactivity with recombinant human (rh) CA5B is observed and less than 1% cross-reactivity with rhCA2 and rhCA7.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Carbonic Anhydrase VA/CA5A by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Carbonic Anhydrase VA/CA5A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3049) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Carbonic Anhydrase VA/CA5A at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Carbonic Anhydrase VA/CA5A in Human Liver. Carbonic Anhydrase VA/CA5A was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human Carbonic Anhydrase VA/CA5A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3049) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocyte cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
