Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [2]
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Validation data
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- Product number
- PA5-26129 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DIPA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references CCDC85B promotes non-small cell lung cancer cell proliferation and invasion.
Feng Y, Gao Y, Yu J, Jiang G, Zhang X, Lin X, Han Q, Rong X, Xu H, Li Q, Qiu X, Wang E
Molecular carcinogenesis 2019 Jan;58(1):126-134
Molecular carcinogenesis 2019 Jan;58(1):126-134
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a DIPA polyclonal antibody (Product # PA5-26129) in HepG2 cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human skeletal muscle tissue using a DIPA polyclonal antibody (Product # PA5-26129), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HepG2 cells using a DIPA polyclonal antibody (Product # PA5-26129) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CCDC85B upregulated active beta-catenin, Wnt targets c-myc, cyclin D1, and MMP-7. WB analysis showed that CCDC85B overexpression downregulated the phosphorylated beta-catenin and upregulated the phosphorylated GSK3beta, active beta-catenin, and its downstream factors: c-myc, cyclin D1, and MMP-7 in A549 and H1299 cells (A). CCDC85B knockdown displayed opposite effects (B). Immunofluorescence assays showed that CCDC85B overexpression upregulated the nuclear beta-catenin in A549 and H1299 cells (C, D)