AP11886a

antibody from Abgent

Targeting: ZBTB24 BIF1, KIAA0441, PATZ2, ZNF450

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP11886a

Provider

Abgent

Proper citation

Abgent Cat#AP11886a, RRID:AB_10819908

Product name

ZBT24 Antibody (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

ZBT24 Antibody (N-term) (Cat. #AP11886a) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the ZBT24 antibody detected the ZBT24 protein (arrow).

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"ZBT24 Antibody (N-term) (Cat. #AP11886a) western blot analysis in ICF, GM12878 and MCF-7 cell line lysates.This demonstrates the ZBT24 antibody detected endogenous ZBT24 protein in MCF-7 and GM12878 cell lines but not in GM08714 cell line (kindly supplied by Dr. Grimmer)."

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

ZBT24 Antibody (N-term) (Cat. #AP11886a) pulled down ZBT24(ZNF450) protein in HepG 2 cell line (kindly supplied by Dr. Grimmer).

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

ZBT24 Antibody (N-term) (Cat. #AP11886a)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ZBT24 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:10~50