MA5-27615

antibody from Invitrogen Antibodies

Targeting: PCDHGA8 KIAA0327, PCDH-GAMMA-A8

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

MA5-27615

Provider

Invitrogen Antibodies

Product name

Protocadherin Gamma Monoclonal Antibody (S159-5)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

S159-5

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Protocadherin Gamma in rat brain membrane with 10 µg of sample. The sample was incubated with Protocadherin Gamma monoclonal antibody (Product # MA5-27615) using a dilution of 1:1000 (1 hour at RT), followed by Goat Anti-Mouse HRP (1 hour at RT) at a dilution of 1:200.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Protocadherin Gamma in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with Protocadherin Gamma monoclonal antibody (Product # MA5-27615) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:200, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) Protocadherin Gamma (pan) Antibody, D) Merged image. Magnification: 60x.