Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ABIN954049 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Protocadherin gamma Subfamily A, 8 (PCDHGA8) (C-Term), (AA 788-816) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 788-816 amino acids from the C-terminal region of Human PCDHGA8
- Description
- Protein A column, followed by peptide affinity purification
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- C-Term,AA 788-816
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Comparative DNA sequence analysis of mouse and human protocadherin gene clusters.
Cadherin superfamily genes: functions, genomic organization, and neurologic diversity.
Large exons encoding multiple ectodomains are a characteristic feature of protocadherin genes.
Phylogenetic analysis of the cadherin superfamily allows identification of six major subfamilies besides several solitary members.
A striking organization of a large family of human neural cadherin-like cell adhesion genes.
Wu Q, Zhang T, Cheng JF, Kim Y, Grimwood J, Schmutz J, Dickson M, Noonan JP, Zhang MQ, Myers RM, Maniatis T
Genome research 2001 Mar;11(3):389-404
Genome research 2001 Mar;11(3):389-404
Cadherin superfamily genes: functions, genomic organization, and neurologic diversity.
Yagi T, Takeichi M
Genes & development 2000 May 15;14(10):1169-80
Genes & development 2000 May 15;14(10):1169-80
Large exons encoding multiple ectodomains are a characteristic feature of protocadherin genes.
Wu Q, Maniatis T
Proceedings of the National Academy of Sciences of the United States of America 2000 Mar 28;97(7):3124-9
Proceedings of the National Academy of Sciences of the United States of America 2000 Mar 28;97(7):3124-9
Phylogenetic analysis of the cadherin superfamily allows identification of six major subfamilies besides several solitary members.
Nollet F, Kools P, van Roy F
Journal of molecular biology 2000 Jun 9;299(3):551-72
Journal of molecular biology 2000 Jun 9;299(3):551-72
A striking organization of a large family of human neural cadherin-like cell adhesion genes.
Wu Q, Maniatis T
Cell 1999 Jun 11;97(6):779-90
Cell 1999 Jun 11;97(6):779-90
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of PCDHGA8 Antibody (C-term) Cat.-No AP53215PU-N in K562 cell line lysates (35ug/lane). This demonstrates the PCDHGA8 antibody detected the PCDHGA8 protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of PCDHGA8 Antibody (C-term) Cat.-No AP53215PU-N with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue reacted with PCDHGA8 Antibody (C-term) Cat.-No AP53215PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.