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antibody from Invitrogen Antibodies
Targeting: FIG4
ALS11, CMT4J, dJ249I4.1, hSac3, KIAA0274, SAC3
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-22083 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FIG4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa , HCT116. Predicted reactivity: Mouse (95%), Rat (93%), Dog (98%), Pig (97%), Chicken (90%), Rhesus Monkey (99%), Chimpanzee (99%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FIG4 Polyclonal Antibody (Product # PA5-22083). Sample (30 µg of whole cell lysate). Lane A: HCT116. 7.5% SDS PAGE. FIG4 Polyclonal Antibody (Product # PA5-22083) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of FIG4 showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in ice-cold MeOH for 5 min and stained using a FIG4 polyclonal antibody (Product # PA5-22083) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIG4 Polyclonal Antibody detects FIG4 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: FIG4 protein stained by FIG4 Polyclonal Antibody (Product # PA5-22083) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using FIG4 (Product # PA5-22083) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
