Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-71133 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FAM214B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This target displays homology in the following species: Cow: 92%; Dog: 92%; Guinea Pig: 92%; Horse: 92%; Human: 100%; Mouse: 92%; Rabbit: 92%; Rat: 92%; Yeast: 77%
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5-1.0 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Hsa_circ_0102171 aggravates the progression of cervical cancer through targeting miR-4465/CREBRF axis.
Tang X, Wen X, Li Z, Wen D, Lin L, Liu J, Li M
Journal of cellular physiology 2021 Jul;236(7):4973-4984
Journal of cellular physiology 2021 Jul;236(7):4973-4984
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FAM214B on human Hela cells. The sample was probed with a FAM214B polyclonal antibody (Product # PA5-71133) using a primary antibody dilution of 1.0 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 5 Figure CREBRF was a downstream target of miR-4465 and its silencing restrained the progression of CC. (a) Intersection of Venn diagram showed four potential genes targeted by miR-4465. (b,c) RT-qPCR results and western blots of KIAA2013, CREBRF, PALM2, and FAM214B mRNA and protein levels under miR-4465 overexpression in CC cells. (d,e) RT-qPCR data for CREBRF expression in CC tissues and cells. (f) Pearson's correlation analysis illustrated CREBRF expression was positively correlated with circ_0102171 and negatively correlated with miR-4465 in 40 CC samples. (g) RT-qPCR tested circ_0102171, miR-4465, and CREBRF mRNA enrichments in Ago2 RIP versus IgG. (h) Potential miR-4465 binding sites in CREBRF 3'-UTR were predicted by starBase and CREBRF-Mut with the mutated sites was designed. (i) Quantification of CREBRF-WT/Mut luciferase activity under miR-4465 mimic transfection. (j) RT-qPCR and western blot results displayed that CREBRF was effectively knocked down in CC cells. (k) CCK-8 reflected SiHa cell proliferation under CREBRF depletion. (l) Flow cytometry analysis for apoptotic SiHa cells under CREBRF depletion. (m) Western blot of cleaved-caspase 3/9, total caspase 3/9, and Bax in SiHa cells under CREBRF silence. (n) Image of transwell migration assay for SiHa cells with CREBRF downregulation. Scale bar = 100 mum. 3'-UTR, 3'-untranslated region; CC, cervical cancer; CCK-8, cell counting kit-8; CREBRF, CREB3 regulatory factor; GAPDH, glyceraldehyde 3-phosphate dehydrogenase