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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-35077 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COL8A2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Start codon disruption with CRISPR/Cas9 prevents murine Fuchs' endothelial corneal dystrophy.
Uehara H, Zhang X, Pereira F, Narendran S, Choi S, Bhuvanagiri S, Liu J, Ravi Kumar S, Bohner A, Carroll L, Archer B, Zhang Y, Liu W, Gao G, Ambati J, Jun AS, Ambati BK
eLife 2021 Jun 8;10
eLife 2021 Jun 8;10
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of COL8A2 in CEM cell lysate (35 µg/lane) using a COL8A2 polyclonal antibody (Product # PA5-35077).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of COL8A2 in HepG2 cells using a COL8A2 polyclonal antibody (Product # PA5-35077) followed by detection using a fluorescent conjugated secondary antibody (green). Nuclei were stained with Dapi (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of COL8A2 in formalin-fixed, paraffin-embedded human kidney tissue using a COL8A2 polyclonal antibody (Product # PA5-35077) followed by peroxidase conjugation of the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3. Ad-Cas9-Col8a2gRNA reduces COL8A2 expression in mouse corneal endothelium but not epithelium. COL8A2 protein immunostaining from the cornea 2 months after injection with Dulbecco's phosphate-buffered saline (DPBS) (4 ul, upper figures) or Ad-Cas9-Col8a2gRNA (0.63 x 10 7 vg in 4 ul, lower figures). In Ad-Cas9-Col8a2gRNA-injected corneas, lower COL8A2 protein expression was seen in corneal endothelium, but not in epithelium. Epi: epithelium, Str: stroma, En (arrow): endothelium. Scale bar = 100 um. Figure 3--figure supplement 1. COL8A2 reduction in corneal endothelium is correlated with the amount of Ad-Cas9-Col8a2gRNA. ( a ) COL8A2 immunostaining in each amount of Ad-Cas9-Col8a2gRNA injection. ( b ) COL8A2 expression in corneal endothelium and corneal epithelium was quantified from the images. The staining intensity in isotype control was subtracted as a background. ( c ) The ratio of COL8A2 staining between corneal endothelium and epithelium. *p
