C1926

antibody from MilliporeSigma / Merck KGaA

Targeting: COL4A5 ASLN, ATS

ELISA (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Protein array (Recommended by provider)

Antibody Data

Product number

C1926

Provider

MilliporeSigma / Merck KGaA

Product name

Monoclonal Anti-Collagen, Type IV antibody produced in mouse

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

COL-94

Storage

Store at -20°C

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 219, 111, 83, 48, 32, 26, 17

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 168

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human heart muscle shows strong membranous positivity in myocytes.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.