40-5000

antibody from Invitrogen Antibodies

Targeting: GJA10 CX62

Western blot (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

40-5000

Provider

Invitrogen Antibodies

Product name

GJA10 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

40-5000 was used in immunofluorescence analysis of Connexin57 (Cx57) on sections of adult mouse retina.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

0.25 mg/mL

Storage

-20°C

References

Submitted references

Connexin 57 is expressed by the axon terminal network of B-type horizontal cells in the rabbit retina.

Pan F, Keung J, Kim IB, Snuggs MB, Mills SL, O'Brien J, Massey SC
The Journal of comparative neurology 2012 Jul 1;520(10):2256-74

---

Connexin-mediated communication controls cell proliferation and is essential in retinal histogenesis.

Kihara AH, Santos TO, Osuna-Melo EJ, Paschon V, Vidal KS, Akamine PS, Castro LM, Resende RR, Hamassaki DE, Britto LR
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience 2010 Feb;28(1):39-52

---

Lack of photoreceptor signaling alters the expression of specific synaptic proteins in the retina.

Kihara AH, Santos TO, Paschon V, Matos RJ, Britto LR
Neuroscience 2008 Feb 19;151(4):995-1005

---

Spatial relationships of connexin36, connexin57 and zonula occludens-1 in the outer plexiform layer of mouse retina.

Ciolofan C, Lynn BD, Wellershaus K, Willecke K, Nagy JI
Neuroscience 2007 Aug 24;148(2):473-88

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of frozen mouse retina using Zymed Rb anti-Connexin 57 (Mid) (Product # 40-5000). Image courtesy of James I. Nagy, PhD, University of Manitoba, Canada.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of frozen mouse retina using Zymed Rb anti-Connexin 57 (Mid) (Product # 40-5000). Image courtesy of James I. Nagy, PhD, University of Manitoba, Canada.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Connexin57 (Cx57) was performed on sections of adult mouse retina. Tissue sections on slides were probed for 24 h at 4°C in a humidified chamber with rabbit polyclonal anti-Cx57 (Product # 40-5000) at an antibody concentration of 1-2 µg/mL diluted in 50 mM Tris-HCl, pH 7.4, containing 1.5% NaCl, 0.3% Triton X-100 (TBST) and 4% normal goat serum. After overnight incubation, sections were washed extensively for 1 h in TBST, and detection of primary antibody was performed for 1.5 h at room temperature with AlexaFluor-488-conjugated donkey anti-rabbit diluted 1:600 in TBST. Sections were then washed in TBST, then in TBS (without triton) and then coversliped with anti-fade medium. Images were taken on a Zeiss 710 confocal microscope at x40 objective magnification, and show immunofluorescence labelling of Cx57 localized at gap junctions between processes of horizontal cells in the outer plexiform layer of adult mouse retina. Data courtesy of Dr. James Nagy's lab.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL