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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB110-96414 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-96414, RRID:AB_1290842
- Product name
- Rabbit Polyclonal DIO3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Hamster
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Decreased translation of Dio3 mRNA is associated with drug-induced hepatotoxicity.
Neuronal hypoxia induces Hsp40-mediated nuclear import of type 3 deiodinase as an adaptive mechanism to reduce cellular metabolism.
The thyroid hormone-inactivating type III deiodinase is expressed in mouse and human beta-cells and its targeted inactivation impairs insulin secretion.
Candidate placental biomarkers for intrauterine alcohol exposure.
Contributions of HIV infection in the hypothalamus and substance abuse/use to HPT dysregulation.
Paracrine signaling by glial cell-derived triiodothyronine activates neuronal gene expression in the rodent brain and human cells.
Dudek KM, Suter L, Darras VM, Marczylo EL, Gant TW
The Biochemical journal 2013 Jul 1;453(1):71-82
The Biochemical journal 2013 Jul 1;453(1):71-82
Neuronal hypoxia induces Hsp40-mediated nuclear import of type 3 deiodinase as an adaptive mechanism to reduce cellular metabolism.
Jo S, Kalló I, Bardóczi Z, Arrojo e Drigo R, Zeöld A, Liposits Z, Oliva A, Lemmon VP, Bixby JL, Gereben B, Bianco AC
The Journal of neuroscience : the official journal of the Society for Neuroscience 2012 Jun 20;32(25):8491-500
The Journal of neuroscience : the official journal of the Society for Neuroscience 2012 Jun 20;32(25):8491-500
The thyroid hormone-inactivating type III deiodinase is expressed in mouse and human beta-cells and its targeted inactivation impairs insulin secretion.
Medina MC, Molina J, Gadea Y, Fachado A, Murillo M, Simovic G, Pileggi A, Hernández A, Edlund H, Bianco AC
Endocrinology 2011 Oct;152(10):3717-27
Endocrinology 2011 Oct;152(10):3717-27
Candidate placental biomarkers for intrauterine alcohol exposure.
Shukla PK, Sittig LJ, Ullmann TM, Redei EE
Alcoholism, clinical and experimental research 2011 Mar;35(3):559-65
Alcoholism, clinical and experimental research 2011 Mar;35(3):559-65
Contributions of HIV infection in the hypothalamus and substance abuse/use to HPT dysregulation.
Langford D, Baron D, Joy J, Del Valle L, Shack J
Psychoneuroendocrinology 2011 Jun;36(5):710-9
Psychoneuroendocrinology 2011 Jun;36(5):710-9
Paracrine signaling by glial cell-derived triiodothyronine activates neuronal gene expression in the rodent brain and human cells.
Freitas BC, Gereben B, Castillo M, Kalló I, Zeöld A, Egri P, Liposits Z, Zavacki AM, Maciel RM, Jo S, Singru P, Sanchez E, Lechan RM, Bianco AC
The Journal of clinical investigation 2010 Jun;120(6):2206-17
The Journal of clinical investigation 2010 Jun;120(6):2206-17
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DIO3 Antibody [NB110-96414] - Detection of Dio3 in rat placenta
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DIO3 Antibody [NB110-96414] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human placenta using 1:50 dilution of DIO3 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining in decidual cells was observed. Staining was performed by Histowiz.
