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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- TA502989 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502989, RRID:AB_11139717
- Product name
- PDF mouse monoclonal antibody, clone OTI3C12 (formerly 3C12)
- Antibody type
- Monoclonal
- Description
- PDF mouse monoclonal antibody, clone OTI3C12 (formerly 3C12)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- Isotype
- IgG
- Antibody clone number
- OTI3C12
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDF (RC205788, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDF.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PDF monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-PDF mouse monoclonal antibody (TA502989) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDF(RC205788 ).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC205788 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDF antibody(TA502989), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-PDF antibody(TA502989),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
