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- Product number
- PA5-101443 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATP5S Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Decreased PGC1β expression results in disrupted human erythroid differentiation, impaired hemoglobinization and cell cycle exit.
Sen T, Chen J, Singbrant S
Scientific reports 2021 Aug 24;11(1):17129
Scientific reports 2021 Aug 24;11(1):17129
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATP5S in HT-29 cells. Samples were incubated with ATP5S polyclonal antibody (Product # PA5-101443).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ATP5S in HT29. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with ATP5S polyclonal antibody (Product # PA5-101443) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 PGC1beta knock-down results in deregulated hemoglobinization of erythroid progenitors. ( A ) Representative flow cytometry plots of bone marrow derived erythroid progenitors within GPA + (SCR). ( B , C ) RT-qPCR analysis of gene expression relative to GAPDH on day 18 of genes important for Oxidative phosphorylation (OXPHOS) in bone marrow derived ( B ) polychromatic erythroblasts and ( C ) orthochromatic erythroblasts respectively (n = SCR:4, Sh3:12, Sh5:6). ( D ) Representative flow cytometry plots of cord blood derived erythroid progenitors within GPA + (SCR). ( E , F ) RT-qPCR analysis of gene expression relative to GAPDH on day 16 of genes important for Oxidative phosphorylation (OXPHOS) in cord blood derived ( E ) polychromatic erythroblasts and ( F ) orthochromatic erythroblasts respectively (n = 4). ( G ) Mitochondria related protein levels were evaluated by western blot in sorted cord blood derived polychromatic erythroblasts from day 16 of culture (ALAS2 (65 kDa) specific bands appear at 120 kDa, likely due to protein dimers). Western blot quantification of ( H ) TOM20 relative to beta-Actin as a marker for mitochondrial mass in relation to cell number, and ( I ) OXPHOS related ATP5S and NDUFA1 relative to TOM20/beta-Actin to account for differences in mitochondrial mass. Expression depicted as normalized to control (SCR, n = 4). ( J ) Quantification of mitochondrial membrane potential of cord blood derived polychromatic erythroblasts measured on day 16 of d
