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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- TA500532 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500532, RRID:AB_10595262
- Product name
- TMEM100 mouse monoclonal antibody, clone OTI10G3 (formerly 10G3)
- Antibody type
- Monoclonal
- Description
- TMEM100 mouse monoclonal antibody, clone OTI10G3 (formerly 10G3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- TMEM100
- Isotype
- IgG
- Antibody clone number
- OTI10G3
- Vial size
- 100 µl
- Concentration
- 0.4 mg/ml
Submitted references Transcriptome profiling-based identification of prognostic subtypes and multi-omics signatures of glioblastoma.
Park J, Shim JK, Yoon SJ, Kim SH, Chang JH, Kang SG
Scientific reports 2019 Jul 22;9(1):10555
Scientific reports 2019 Jul 22;9(1):10555
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TMEM100 (RC203980, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TMEM100.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-TMEM100 mouse monoclonal antibody (TA500532) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TMEM100(RC203980).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TMEM100 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500532)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TMEM100 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500532)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-TMEM100 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500532)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TMEM100 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500532)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-TMEM100 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500532)
- Validation comment
- IHC
