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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- MA5-24949 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TMEM100 Monoclonal Antibody (OTI10G3)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI10G3
- Vial size
- 100 µL
- Concentration
- 0.4 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references TMEM100 induces cell death in non‑small cell lung cancer via the activation of autophagy and apoptosis.
He Q, Dong Y, Zhu Y, Ding Z, Zhang X, Wang Z, Ai R, He Y
Oncology reports 2021 May;45(5)
Oncology reports 2021 May;45(5)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TMEM100 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with TMEM100 (Product # MA5-24949) monoclonal antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TMEM100 in COS7 cells. Cells were transfected with a plasmid overexpressing TMEM100 and probed with a TMEM100 monoclonal antibody (Product # MA5-24949).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human pancreas tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a TMEM100 monoclonal antibody (Product # MA5-24949).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human kidney tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a TMEM100 monoclonal antibody (Product # MA5-24949).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human endometrium tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a TMEM100 monoclonal antibody (Product # MA5-24949).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human colon tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a TMEM100 monoclonal antibody (Product # MA5-24949).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human ovary tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a TMEM100 monoclonal antibody (Product # MA5-24949).
