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LearnABIN1580461
antibody from antibodies-online
Targeting: UBQLN2
Chap1, CHAP1/DSK2, Dsk2, LIC-2, N4BP4, PLIC-2, PLIC2, RIHFB2157
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- ABIN1580461 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Ubiquilin 2 (UBQLN2) antibody
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- affinity purified antibody
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6H9
- Vial size
- 100 μL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term or -20°C long term.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Pattern of ubiquilin pathology in ALS and FTLD indicates presence of C9ORF72 hexanucleotide expansion.
A hexanucleotide repeat expansion in C9ORF72 is the cause of chromosome 9p21-linked ALS-FTD.
Expanded GGGGCC hexanucleotide repeat in noncoding region of C9ORF72 causes chromosome 9p-linked FTD and ALS.
Ubiquilin functions in autophagy and is degraded by chaperone-mediated autophagy.
PLIC proteins or ubiquilins regulate autophagy-dependent cell survival during nutrient starvation.
Brettschneider J, Van Deerlin VM, Robinson JL, Kwong L, Lee EB, Ali YO, Safren N, Monteiro MJ, Toledo JB, Elman L, McCluskey L, Irwin DJ, Grossman M, Molina-Porcel L, Lee VM, Trojanowski JQ
Acta neuropathologica 2012 Jun;123(6):825-39
Acta neuropathologica 2012 Jun;123(6):825-39
A hexanucleotide repeat expansion in C9ORF72 is the cause of chromosome 9p21-linked ALS-FTD.
Renton AE, Majounie E, Waite A, Simón-Sánchez J, Rollinson S, Gibbs JR, Schymick JC, Laaksovirta H, van Swieten JC, Myllykangas L, Kalimo H, Paetau A, Abramzon Y, Remes AM, Kaganovich A, Scholz SW, Duckworth J, Ding J, Harmer DW, Hernandez DG, Johnson JO, Mok K, Ryten M, Trabzuni D, Guerreiro RJ, Orrell RW, Neal J, Murray A, Pearson J, Jansen IE, Sondervan D, Seelaar H, Blake D, Young K, Halliwell N, Callister JB, Toulson G, Richardson A, Gerhard A, Snowden J, Mann D, Neary D, Nalls MA, Peuralinna T, Jansson L, Isoviita VM, Kaivorinne AL, Hölttä-Vuori M, Ikonen E, Sulkava R, Benatar M, Wuu J, Chiò A, Restagno G, Borghero G, Sabatelli M, ITALSGEN Consortium, Heckerman D, Rogaeva E, Zinman L, Rothstein JD, Sendtner M, Drepper C, Eichler EE, Alkan C, Abdullaev Z, Pack SD, Dutra A, Pak E, Hardy J, Singleton A, Williams NM, Heutink P, Pickering-Brown S, Morris HR, Tienari PJ, Traynor BJ
Neuron 2011 Oct 20;72(2):257-68
Neuron 2011 Oct 20;72(2):257-68
Expanded GGGGCC hexanucleotide repeat in noncoding region of C9ORF72 causes chromosome 9p-linked FTD and ALS.
DeJesus-Hernandez M, Mackenzie IR, Boeve BF, Boxer AL, Baker M, Rutherford NJ, Nicholson AM, Finch NA, Flynn H, Adamson J, Kouri N, Wojtas A, Sengdy P, Hsiung GY, Karydas A, Seeley WW, Josephs KA, Coppola G, Geschwind DH, Wszolek ZK, Feldman H, Knopman DS, Petersen RC, Miller BL, Dickson DW, Boylan KB, Graff-Radford NR, Rademakers R
Neuron 2011 Oct 20;72(2):245-56
Neuron 2011 Oct 20;72(2):245-56
Ubiquilin functions in autophagy and is degraded by chaperone-mediated autophagy.
Rothenberg C, Srinivasan D, Mah L, Kaushik S, Peterhoff CM, Ugolino J, Fang S, Cuervo AM, Nixon RA, Monteiro MJ
Human molecular genetics 2010 Aug 15;19(16):3219-32
Human molecular genetics 2010 Aug 15;19(16):3219-32
PLIC proteins or ubiquilins regulate autophagy-dependent cell survival during nutrient starvation.
N'Diaye EN, Kajihara KK, Hsieh I, Morisaki H, Debnath J, Brown EJ
EMBO reports 2009 Feb;10(2):173-9
EMBO reports 2009 Feb;10(2):173-9
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of untransfected primary mouse neuron and glia cell cultures (lane 1), the same cells transduced with human ubiquilin 2 wild type (lane 2), with ubiquilin 2 P506T mutant (lane 3), with ubiquilin 2 P497S mutant (lane 4) and with enhanced GFP control (lane 5), all probed with ABIN1580461. Also seen is ABIN1580461 staining in HeLa cells (lane 6) and 3T3 cells (lane 7). In primary mouse neuron and glia cell culture, endogenous ubiquilin 2 appears as a weak band at 68 kDa in all transduced and non-transduced cells, indicating low endogenous expression of mouse ubiquilin 2. Strong bands are seen in the cells transduced with human wild type or mutant ubiquilin 2. Small proteins run at 50 kDa in these cells are the fragments of ubiquilin 2. Note, ubiquilin 2 run at ~66 kDa in human HeLa cells and 68 kDa in rodent 3T3 cells.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- HeLa cell cultures stained with ABIN1580461 (green) and ?s chicken polyclonal antibody to vimentin: CPCA-Vim (red). In most individual cells ubiquilin 2 is present diffusely in the cytoplasm of cells, though some cells show enrichment of the protein in spherical autophagosome-like structure.
