Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- PA5-21097 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LRRTM2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is SK-N-SH cell lysate.
- Concentration
- 1 mg/mL
Submitted references A model for regulation by SynGAP-α1 of binding of synaptic proteins to PDZ-domain 'Slots' in the postsynaptic density.
Walkup WG, Mastro TL, Schenker LT, Vielmetter J, Hu R, Iancu A, Reghunathan M, Bannon BD, Kennedy MB
eLife 2016 Sep 13;5
eLife 2016 Sep 13;5
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of LRRTM2 in SK-N-SH cell lysate with LRRTM2 Polyclonal Antibody (Product # PA5-21097) at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of LRRTM2 in human brain tissue with LRRTM2 Polyclonal Antibody (Product # PA5-21097) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of LRRTM2 in human brain tissue with LRRTM2 Polyclonal Antibody (Product # PA5-21097) at 2.5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 11. Altered composition of the PSD in mice with heterozygous deletion of synGAP . Ratios of amounts of the indicated proteins to PSD-95 in each lane were measured as described in 'Materials and methods' and are reported as mean +- S.E. For all blots except those for neuroligin-1, PSD-95 was detected with a secondary Ab labeled with AlexaFluor680 and the binding protein was detected with secondary Ab labeled with IRDye 800. On the neuroligin-1 blot, both PSD-95 and neuroligin-1 were detected with AlexaFluor680; the two bands were well-separated in each lane. Representative sets of visualized bands for WT and HET from the same blot are shown below the graphs. Individual points represent the ratio of the indicated protein to PSD-95 in a single lane (n refers to these technical replicates). The WT and HET PSD preparations were each made from six animals; thus, each of the two preparations represents six biological replicates. ( A ) SynGAP to PSD-95 ratio. Data were collected for 22 lanes from two blots containing 5 ug total PSD fraction per lane. One blot contained six lanes WT and six lanes HET samples, the other contained five lanes of each. The mean ratio of synGAP to PSD-95 was 0.234 +- 0.012 for WT (n = 11) and 0.179 +- 0.005 (n = 11) for HET (-24%). Means were compared by unpaired, one-tailed t-test with Welch correction, p = 0.0007. Effect size, d = 1.75. ( B ) TARP g -2,3,4,8 to PSD-95 ratio. Data were collected for 24 lanes from two blots containing 10 u