IC6030A

antibody from R&D Systems

Targeting: IDO1 IDO, INDO

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC6030A

Provider

R&D Systems

Product name

Human Indoleamine 2,3-dioxygenase/IDO APC-conjugated Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Indoleamine 2,3-dioxygenase/IDO in direct ELISA.

Reactivity

Human

Host

Mouse

Conjugate

Red dye

Antigen sequence

P14902


Isotype

IgG

Antibody clone number

700838

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

References

Submitted references

Trichinella spiralis Excretory-Secretory Products Induce Tolerogenic Properties in Human Dendritic Cells via Toll-Like Receptors 2 and 4.

Ilic N, Gruden-Movsesijan A, Cvetkovic J, Tomic S, Vucevic DB, Aranzamendi C, Colic M, Pinelli E, Sofronic-Milosavljevic L
Frontiers in immunology 2018;9:11

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Monocyte Differentiation towards Protumor Activity Does Not Correlate with M1 or M2 Phenotypes.

Chimal-Ramírez GK, Espinoza-Sánchez NA, Chávez-Sánchez L, Arriaga-Pizano L, Fuentes-Pananá EM
Journal of immunology research 2016;2016:6031486

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1-Methyl-tryptophan attenuates regulatory T cells differentiation due to the inhibition of estrogen-IDO1-MRC2 axis in endometriosis.

Wei C, Mei J, Tang L, Liu Y, Li D, Li M, Zhu X
Cell death & disease 2016 Dec 1;7(12):e2489

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Indoleamine 2,3-dioxygenase/IDO in Human MDSCs by Flow Cytometry. Human PBMC-derived myeloid-derived suppressor cells (MDSCs) treated with 10 ng/mL Recombinant Human IL-6 (Catalog # 206-IL) and 10 ng/mL Recombinant Human GM-CSF (Catalog # 215-GM) for 7 days were stained with Mouse Anti-Human Siglec-3/CD33 PE-conjugated Monoclonal Antibody (Catalog # FAB1137P) and either (A) Mouse Anti-Human Indoleamine 2,3-dioxygenase/IDO APC-conjugated Monoclonal Antibody (Catalog # IC6030A) or (B) Mouse IgG₁ Allophycocyanin Isotype Control (Catalog # IC002A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.