H00003620-M05

antibody from Abnova Corporation

Targeting: IDO1 IDO, INDO

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003620-M05

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003620-M05, RRID:AB_606454

Product name

IDO1 monoclonal antibody (M05), clone 4D2

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant IDO1.

Antigen sequence

MAHAMENSWTISKEYHIDEEVGFALPNPQENLPDF
YNDWMFIAKHLPDLIESGQLRERVEKLNMLSIDHL
TDHKSQRLARLVLGCITMAYVWGKGHGDVRKVLPR
NIAVPYCQLSKKLELPPILVYADCVLANWKKKDPN
KPLTYENMDVLFSFRDGDCSKGFFLVSLLVEIAAA
SAIKVIPTVFKAMQMQERDTLLKALLEIASCLEKA
LQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSD
GLVYEGFWEDPKEFAGGSAGQSSVFQCFDVLLGIQ
QTAGGGHAAQFLQDMRRYMPPAHRNFLCSLESNPS
VREFVLSKGDAGLREAYDACVKALVSLRSYHLQIV
TKYILIPASQQPKENKTSEDPSKLEAKGTGGTDLM
NFLKTVRSTTEKSLLKEG


Isotype

IgG

Antibody clone number

4D2

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

IDO1 monoclonal antibody (M05), clone 4D2 Western Blot analysis of IDO1 expression in HeLa ( Cat # L013V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of IDO1 expression in transfected 293T cell line by IDO1 monoclonal antibody (M05), clone 4D2.Lane 1: IDO1 transfected lysate (Predicted MW: 45.3 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged IDO1 is 0.3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to IDO1 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol