695002

antibody from BioLegend

Targeting: IDO1 IDO, INDO

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

695002

Provider

BioLegend

Proper citation

BioLegend Cat#695002, RRID:AB_2650745

Product name

Purified anti-IDO1

Antibody type

Monoclonal

Reactivity

Human

Host

Rat

Conjugate

Unconjugated

Antibody clone number

W16073A

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysates (15 µg protein) from non-treated and recombinant human IFN-γ treated (50 ng/mL, 16 hours) HeLa were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 0.5 µg/mL purified anti-IDO1 (clone W16073A) antibody. Proteins were visualized using an HRP goat anti-rat-IgG secondary antibody (clone Poly4054) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control.

Conjugate

Unconjugated