Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-141130 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IDO Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IDO in human placenta tissue lysate. Samples were incubated in IDO Polyclonal antibody (Product # PA5-141130) using a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of IDO in HeLa cells (green). Samples were incubated in IDO Polyclonal antibody (Product # PA5-141130). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of IDO in HUVEC cells (green). Samples were incubated in IDO Polyclonal antibody (Product # PA5-141130). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of IDO in paraffin-embedded human spleen tissue. Samples were incubated in IDO Polyclonal antibody (Product # PA5-141130). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of IDO in paraffin-embedded human tonsil tissue. Samples were incubated in IDO Polyclonal antibody (Product # PA5-141130). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of IDO in HUVEC cells. Samples were incubated in IDO Polyclonal antibody (Product # PA5-141130) using a dilution of 1:100 (red). Unlabeled control (cells without incubation with primary antibody; black).