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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- 712023 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ULBP3 Recombinant Polyclonal Antibody (11HCLC)
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- This antibody is predicted to react with Monkey, Horse.
- Antibody clone number
- 11HCLC
- Concentration
- 0.5 mg/mL
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- For immunofluorescence analysis, GTL-16 cells were fixed and permeabilized for detection of endogenous ULBP3 using Anti-ULBP3 Recombinant Rabbit Polyclonal Antibody (Product # 712023, 1:100) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Panel a-d) clearly demonstrates membrane localization of ULBP3 in GTL-16 cells. Panel e-h) shows no specific staining in A549 cells which is ULBP3 negative, demonstrating specificity. The images were captured at 60X magnification.
