- PA5-78234 - Invitrogen Antibodies ZFX antibody

Song N, Zhang Y, Kong F, Yang H, Ma X
Cancer cell international 2020;20:359

Qin X, Cui X
3 Biotech 2020 Apr;10(4):187

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: ZFX Polyclonal Antibody detects ZFX protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZFX Polyclonal Antibody (Product # PA5-78234) diluted at 1:1,000.

Supportive validation

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 4 ZFX and YKL-40 are involved in the HOXA-AS2-miR-302c-3p regulatory axis. a the target site of ZFX and relative miR-302c-3p was confirmed by Target Scan. b , c ZFX and YKL-40 mRNA and proteins levels in endometrial cancer tissues versus normal endometrial tissues measured by qRT-PCR and western blotting. d ZFX and YKL-40 levels in HOXA-AS2-modified Ishikawa cells measured by qRT-PCR and western blotting. e ZFX and YKL-40 levels in miR-302c-3p-modified Ishikawa cells measured by qRT-PCR and western blotting. Data are the mean +- SD from independent experiments. * P < 0.05, ** P < 0.01 *** P < 0.001

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 6 YKL-40 promotes the development of type I endometrial cancer cells and is regulated by ZFX in vitro. a Cell proliferation of YKL-40-modified Ishikawa cells was examined by CCK-8 assay. b Cell cycle phases of YKL-40-modified Ishikawa cells were examined by flow cytometry. c Fractions of apoptotic YKL-40-modified Ishikawa cells were examined by flow cytometry. d Invasion of miR-302c-3p-modified cells was examined by Transwell invasion assays. e , f YKL-40 mRNA and protein levels in ZFX-modified Ishikawa cells were measured by qRT-PCR and western blotting. Data are the mean +- SD from independent experiments. ** P < 0.01, *** P < 0.001. g Proposed negative feedback loop mechanism of the HOXA-AS2-miR-302c-3p/ZFX/YKL-40 axis in Ishikawa cells. HOXA-AS2 regulate the biological behavior of the type I endometrium by negatively regulating miR-302c-3p expression. MiR-302c-3p acts as a tumor suppressor in endometrial cancer, low expression of miR-302c-3p promotes the expression of transcription factor ZFX, and high expression of ZFX promotes the expression of hydrolase YKL-40, which in turn promotes malignant behavior of type I endometrium

PA5-78234