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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-54957 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SPAG7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: ADLLGSILSS MEKPPSLGDQ ETRRKAREQA ARLKKLQEQE KQQKVEFRKR MEKEVSDFIQ DSGQIKKKFQ PMNKIERSIL HDVVEVAG
- Concentration
- 0.3 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SPAG7 in control (vector only transfected HEK293T lysate) and SPAG7 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a SPAG7 Polyclonal Antibody (Product # PA5-54957).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SPAG7 in control (vector only transfected HEK293T lysate) and SPAG7 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a SPAG7 Polyclonal Antibody (Product # PA5-54957).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of SPAG7 in human cell line A-431 shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a SPAG7 Polyclonal Antibody (Product # PA5-54957).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SPAG7 in human fallopian tube using a SPAG7 Polyclonal Antibody (Product # PA5-54957) shows cytoplasmic and nuclear positivity in glandular cells.
