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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-27598 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Slo3 Monoclonal Antibody (S2-16)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- S2-16
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Slo3 in rat brain membrane lysate with 15 µg of sample. The sample was blocked with 1.5% BSA, incubated with Slo3 monoclonal antibody (Product # MA5-27598) using a dilution of 1:1000 (2 hours at 4°C), followed by Sheep Anti-Mouse HRP (1 hour at RT).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Slo3 in human HaCaT cells. Sample was fixed with 100% cold methanol (10 minutes at -20°C), incubated with Slo3 monoclonal antibody (Product # MA5-27598) using a dilution of 1:100 (1 hour at RT), and followed by FITC Goat Anti-Mouse (green) secondary antibody at a dilution of 1:50 (1 hour at RT).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Slo3 in paraffin-embedded mouse back skin. Sample was fixed with Bouin's fixative solution, incubated with Slo3 monoclonal antibody (Product # MA5-27598) using a dilution of 1:100 (1 hour at RT), and followed by FITC Goat Anti-Mouse (green) (1 hour at RT) at a dilution of 1:50.
