A03090

antibody from Boster Biological Technology

Targeting: NOX5 NOX5A, NOX5B

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

A03090

Provider

Boster Biological Technology

Product name

Anti-NOX5 Antibody Picoband™

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for NOX5 detection. Tested with WB, ICC/IF, FCM, Direct ELISA in Human.

Reactivity

Human

Host

Rabbit

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of NOX5 using anti-NOX5 antibody (A03090). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate,Lane 2: human placenta tissue lysates,Lane 3: human SGC-7901 whole cell lysate,Lane 4: human Jurkat whole cell lysate,Lane 5: human THP-1 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOX5 antigen affinity purified polyclonal antibody (Catalog # A03090) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOX5 at approximately 86KD. The expected band size for NOX5 is at 86KD.

Additional image