11528-1-AP

antibody from Invitrogen Antibodies

Targeting: SRP14 ALURBP, MGC14326

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11528-1-AP

Provider

Invitrogen Antibodies

Product name

SRP14 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MVLLESEQF LTELTRLFQK CRTSGSVYIT LKKYDGRTKP IPKKGTVEGF EPADNKCLLR ATDGKKKIST VVSSKEVNKF QMAYSNLLRA NMDGLKKRDK KNKTKKTKAA AAAAAAAPAA AATAPTTAAT TAATAAQ (1-136 aa encoded by BC035495)

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.41 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 11528-1-AP (SRP14 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human heart tissue were subjected to SDS PAGE followed by western blot with 11528-1-AP (SRP14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 11528-1-AP (SRP14 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using SRP14 antibody 11528-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using SRP14 antibody 11528-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-SRP14 (IP:11528-1-AP, 4ug; Detection:11528-1-AP 1:300) with A549 cells lysate 1600ug.