PA5-54816

antibody from Invitrogen Antibodies

Targeting: APBB2 FE65L, FE65L1, MGC35575

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-54816

Provider

Invitrogen Antibodies

Product name

APBB2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: SEKLEGKEPH PQDSSSCEIL PSQPRRTKSF LNYYADLETS ARELEQNRGN HHGTAEEKSQ PVQGQASTII GNGDLLLQKP NRPQSSPEDG QVATVSSSPE TKKDHPKTGA KTDCALHRIQ NLAPS

Concentration

0.2 mg/mL

References

Submitted references

miR‑205‑3p promotes lung cancer progression by targeting APBB2.

Xu LB, Xiong J, Zhang YH, Dai Y, Ren XP, Ren YJ, Han D, Wei SH, Qi M
Molecular medicine reports 2021 Aug;24(2)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of APBB2 in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a APBB2 Polyclonal Antibody (Product # PA5-54816). Remaining relative intensity is presented.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of APBB2 in control (vector only transfected HEK293T lysate) and LY406673 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a APBB2 Polyclonal Antibody (Product # PA5-54816).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of APBB2 in human cell line U-251 MG shows positivity in mitochondria. Samples were probed using an APBB2 Polyclonal Antibody (Product # PA5-54816).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of APBB2 in human pancreas shows distinct granular positivity in exocrine cells. Samples were probed using an APBB2 Polyclonal Antibody (Product # PA5-54816).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6. miR-205-3p antagomir inhibits lung cancer growth and promotes apoptosis in mouse xenografts. (A) Representative images of excised xenograft tumors and (B) tumor volumes from mice treated with the antagomir NC or miR-205-3p antagomir. (C) Reverse transcription-quantitative PCR analysis was used to detect miR-205-3p expression in tumors. (D) Western blot analysis was used to detect APBB2, Bax and Bcl-2 protein expression levels in tumors. *P