LS-C397010

antibody from LSBio

Targeting: PLP1 GPM6C, PLP, SPG2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C397010

Provider

LSBio

Product name

PLP1 / Myelin PLP Antibody (aa143-197) LS-C397010

Antibody type

Polyclonal

Description

Protein G purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Short term: store at -20°C. Long term: store at -80°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot. All lanes: PLP1 antibody at 4 ug/ml. Lane 1: HeLa whole cell lysate. Lane 2: HL60 whole cell lysate. Lane 3: K562 whole cell lysate. Lane 4: Thp-1 whole cell lysate. Lane 5: U937 whole cell lysate. Lane 6: A549 whole cell lysate. Lane 7: Caco-2 whole cell lysate. Lane 8: HepG-2 whole cell lysate. Secondary Goat polyclonal to Rabbit IgG at 1:10000 dilution. Predicted band size: 30 kDa. Observed band size: 30 kDa.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot All lanes: PLP1 antibody at 4µg/ml Lane 1: Hela whole cell lysate Lane 2: HL60 whole cell lysate Lane 3: K562 whole cell lysate Lane 4: THP-1 whole cell lysate Lane 5: U937 whole cell lysate Lane 6: A549 whole cell lysate Lane 7: Caco-2 whole cell lysate Lane 8: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 31, 27 kDa Observed band size: 31 kDa

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using antibody at dilution of 1:100.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using antibody at dilution of 1:100.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using antibody at dilution of 1:100.