600-401-J12

antibody from Rockland Immunochemicals, Inc.

Targeting: ARHGAP22 RhoGAP2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-J12

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-J12, RRID:AB_2614372

Product name

Anti-ARHGAP22 pS397 (RABBIT) Antibody - 600-401-J12

Antibody type

Polyclonal

Vial size

50 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western Blot of Rabbit anti-ARHGAP22 pS397 antibody. Lane 1: NIH3T3 cells transfected with a null vector. Lane 2: NIH3T3 cells transfected with ARHGAP22. Lane 3: NIH3T3 cells transfected with ARHGAP22 S22 to alanine mutation. Lane 4: NIH3T3 cells transfected with ARHGAP22 S397 to alanine mutaion. Primary antibody: Left: ARHGAP22 pS22, Right: ARHGAP22 pS397 antibody at 1µg/mL for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO O/N at 4°C. Predicted/Observed size: 68 kDa for ARHGAP22. Other band(s): Unmodified ARHGAP22. ARHGAP22-pS22 antibody recognizes the S397>A mutation, not the S22>mutation; ARHGAP22 pS397 recognizes the pS22>A mutation, not the pS397>A mutation; Confirms the specificity of each ARHGAP22 phospho specific antibody.

Validation comment

Western Blot